The ladder contains one orange reference band at 70 kDa and a mixture of blue and yellow ribbons at 10 kDa.ġ. The protein marker is supplied in a ready-to-use format for direct loading onto gels no need to heat, reduce, or add sample buffer prior to use.This prestained protein marker is designed for monitoring the progress of SDS-polyacrylamide gel electrophoresis, for assessing transfer efficiency onto PVDF, nylon and nitrocellulose membranes, and for estimating the approximate size of separated proteins that have been made visible with gel stains or Western blot detection reagents. Prestained Protein Marker is a mixture of ten blue, orange or green-stained proteins (10 to 180 kDa) for use as size standards in protein electrophoresis (SDS-PAGE) and western blotting. Membrane-compatible: colored bands transfer to membranes for Western blotting.Two reference bands: orange at 70 kDa and a mixture of blue and yellow ribbons at 10 kDa.Quality tested: each lot evaluated by SDS-PAGE and Western blotting.Sharp bands: color-coded bands of similar intensity for easy visualization.Ready-to-use: supplied in a loading buffer for direct loading on gels no need to boil.Size range: 10 proteins spanning from 10 to 180 kDa.